For E-PG recordings in the protocerebral bridge, we manually drew ROIs on the 16 individual glomeruli visible in each recording (one additional column on either end of the PB does not contain E-PGs). We then paired each ROI on the left side with an ROI on the right side, using a pairing scheme which wrapped on either side independently (i.e. 1L/1R, 8L/2R, 7L/3R, see Figure 10; Figure 10—figure supplement 1D). For each pair, we obtained the time-series for the ROIs across all frames in the recording and found their normalized cross-correlation coefficient at zero lag, ranging from −1 to 1. We plot the coefficient values for each pair (Figure 10—figure supplement 1E) and the mean coefficient across all pairs from all recordings after applying the Fisher z-transformation. We then shifted the pairing scheme by one position on the right side and repeated the procedure until all pairing schemes had been evaluated.

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