In addition to manually drawn ROIs, we generated ROIs based on polarization tuning maps (Figure 1—figure supplement 2). Briefly, we discretized tuning maps so that they contained only six preferred angles of polarization, corresponding to those presented in the tuning experiment ±15°, plus null values for excluded pixels. For each angle, we identified contiguous areas of 20 or more pixels with that tuning and retained the largest area as an ROI.

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