For each pixel, we found the average fluorescence intensity across the frames captured during each angle presentation to obtain a polarization tuning curve. Since a polarization-tuned analyzer should respond identically to parallel angles of polarization (e.g. 0°/180°), we expected bimodal data with diametrically opposite modes. We therefore found the axial mean resultant vector, correcting for grouped data, and took its angle as the preferred angle of polarization, defined modulo 180° (Batschelet, 1965; Berens, 2009; Zar, 1999).

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