To characterize retinotopic responses to unpolarized stimuli, a single bright, vertical bar was presented on the LED display (32 × 1 pixel) with all other LEDs off (0.78 Weber contrast). Bars initially remained stationary for 3 s, then jittered left and right (±1 pixel) for 3 s, followed by an inter-trial period of 4 s with all LEDs off. Bars were presented at five equally spaced azimuth positions spanning ±90°, presented sequentially from left to right around the fly. This procedure was repeated twice.

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