The genetically encoded photosensitizer, miniSOG, was used to ablate specific neurons as previously described (Qi et al., 2012). Synchronized Late L1 larva animals (24 hr after egg hatching at 20°C) expressing miniSOG under specific promoters were exposed to wide-field blue light (460 nm) at an intensity of 57 mW/cm2 for 30 min, and then animals were grown in the 20°C incubator before experiments. The ablation efficiency was measured by comparing mCherry fluorescent signal with and without blue light stimulation.

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