Mating efficiency was assessed as previously described (Yin et al., 2017). Briefly, two young adult stage hermaphrodites from male- or hermaphrodite-conditioned medium were cultured with two young-adult males for 24 hr. Successful mating was scored when more than three male progenies were generated in the mating plate. Mating efficiency was obtained by calculating the percentage of successful mating in more than 15 plates.

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