The aldicarb assay was performed as previously described (Vashlishan et al., 2008). Aldicarb (ApexBio) was dissolved in ethyl alcohol and added to NGM at a final concentration of 1.4 mM (Testing hermaphrodites) or 0.5 mM (Testing males). These plates (35 mm) were seeded with 75 µl OP50 and allowed to dry overnight before use. More than 20 animals at the young adult stage (otherwise indicated) were picked on an aldicarb plate for aldicarb assay. Animals were scored as paralyzed when they did not respond to the platinum wire prodding. The paralyzed animals were counted every 10 or 15 min. At least three double-blind replicates for each group were tested.

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