All worm expression vectors were modified versions of pPD49.26 (A. Fire) or miniMos vector pCFJ910. Standard methods and procedures were utilized for all of the plasmids. A 3.1 kb ceh-36 promoter was used for expression in ASE and AWC chemosensory neurons. A 3 kb odr-10 promoter was used for expression in AWA chemosensory neurons. A 3 kb str-2 promoter was used for expression in AWC chemosensory neurons. A 3 kb str-1 promoter was used for expression in AWB chemosensory neurons. A 3 kb srb-6 promoter was used for expression in ADF, ADL, and ASH chemosensory neurons. A 3 kb gpa-4 promoter was used for expression in ASI chemosensory neurons. A 3 kb gcy-15 promoter was used for expression in ASG chemosensory neurons. A 3.9 kb sra-7 promoter was used for expression in ASK chemosensory neurons. A 4.1 kb flp-21 promoter was used for expression in the majority of the chemosensory neurons. A 4.3 kb acr-5 promoter was used for expression in DB and VB motor neurons. A 2.4 kb myo-3 myosin promoter was used for expression in body muscles. For rescue experiments, TAX-2 (F36F2.5.1), ODR-3 (C34D1.3.1), and ACR-16 (F25G6.3) were amplified from the N2 cDNA library using gene-specific primers.

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