Total RNA was isolated from gonadal adipose tissue (eWAT; n = 9) samples using the Ambion RiboPure (Thermo Fisher Scientific). RNA was quantified in a Nanodrop ND-1000 spectrophotometer, and RNA purity and integrity was checked by using a Bioanalyzer-2100 equipment (Agilent Technologies, Inc, Santa Clara, CA). Libraries were prepared using the TruSeq RNA Sample Preparation Kit (Illumina Inc, CA) and were paired-end sequenced (2 × 75 bp), by using the TruSeq SBS Kit v3-HS (Illumina Inc), in a HiSeq 2000 platform (Illumina Inc). More than 30 M PE reads were obtained for all samples.

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