C. elegans were maintained under standard conditions at 20°C on plates made from nematode growth medium (NGM). E. coli OP50 was used as a food source for all experiments except where HB101 E. coli was utilized for electrophysiology study. A description of all alleles can be found at http://www.wormbase.org/#012-34-5. Animals were obtained from Bristol variety N2 strain unless specially indicated. Transgenic animals were prepared by microinjection, and integrated transgenes were isolated following UV irradiation or by miniMos insertion.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.