C. elegans were maintained under standard conditions at 20°C on plates made from nematode growth medium (NGM). E. coli OP50 was used as a food source for all experiments except where HB101 E. coli was utilized for electrophysiology study. A description of all alleles can be found at http://www.wormbase.org/#012-34-5. Animals were obtained from Bristol variety N2 strain unless specially indicated. Transgenic animals were prepared by microinjection, and integrated transgenes were isolated following UV irradiation or by miniMos insertion.

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