Verification on various scalps
This protocol is extracted from research article:
Leaf-inspired homeostatic cellulose biosensors
Sci Adv, Apr 16, 2021; DOI: 10.1126/sciadv.abe7432

Participants included 10 Asians and 8 Westerners. Two of the Westerners were women with long hair. In experiments involving the Westerners, impedance and EEG were measured from five channels of CSs (FP1, FP2, Oz, O1, and O2) (fig. S4E). AFz and the right mastoids served as ground and reference electrodes, respectively. Eye open/closed/blink paradigm (eye opening for 10 s, eye closing for 10 s, followed by eye blinking for 10 s) was used. For Asians, impedance and EEG were measured from 10 channels (GSs: FP1, FP2, Oz, O1, and O2; CSs: AF7, AF8, POz, PO7, and PO8) (fig. S4G). The same eye open/closed and SSVEPs paradigms (5, 7.5, and 10 Hz) were used. To quantify the signal quality of the dataset related to SSVEP between the CSs and GSs, Pearson’s correlation coefficient analysis was conducted (fig. S5A). In addition, SNR analysis of SSVEPs for channels positioned to the visual cortex region was performed (GSs: Oz, O1, and O2; CSs: POz, PO7, and PO8). The energy of y(f) was calculated using fast Fourier transform. For SSVEPs at a stimulation frequency f, the SNR in decibels was defined as the ratio of y(f) to the mean value of the two adjacent frequencies as follows

For the SSVEP (5, 7.5, and 10 Hz) paradigm, the grand average SNR of CSs and GSs was 22.701 ± 10.96 and 23.835 ± 9.67, respectively (fig. S5B). There was no significant difference in the two-sample test (P = 0.3659). In the PSD analysis, the peaks corresponding to the stimulation frequencies were clearly detected (fig. S5C).

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