4T1 cells (4 × 105 cells per well) were seeded in six-well plates and cultured for 24 hours. Then, cells were incubated with free PR104A or free CPT (10 μM) for 12 hours (n = 3). After incubation, the cells were washed, collected, and broken by freezing and thawing. The free drug ratio was determined by ultrafiltration.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.