4T1 cells (1000 cells per well) or 3T3 cells (2000 cells per well) were seeded in 96-well plates and cultured under normoxic conditions for 12 hours. Then, the cells were cultured in normoxic or hypoxic atmospheres for another 12 hours. After culture, the cells were incubated with serial dilutions of free CPT, free PR104A, PR104A + CPT mixture, CSSP NPs, or CP NPs in normoxic or hypoxic atmospheres for 48 hours (n = 6). Then, 10 μl of CCK-8 was added into each well and incubated for 2 hours, and the absorbance at 450 nm was measured by using a microplate reader (BioTek, USA).

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