Measurement of electrical resistance, conductivity, and electromechanical stability of the CM
This protocol is extracted from research article:
Leaf-inspired homeostatic cellulose biosensors
Sci Adv, Apr 16, 2021; DOI: 10.1126/sciadv.abe7432

The circular CMs with 3-cm diameter and 20-μm thickness were sandwiched with top and bottom electrodes. A PDMS (Sylgard 184 kit) film was cut with an opening (4 cm2), which served as a well for holding the electrolyte solution. Electrolytes were filled into a molded PDMS well. The electrical resistance was recorded from a CM using a pair of top and bottom electrodes using a multimeter (Keysight 34460A) interfaced with computerized recording software (BenchVue 3.7).

For electrochemical stability tests of the swollen CM, we varied the RH through a homemade humidifier. Briefly, air bubbling through a water-containing conical flask was led into a gas-purge desiccator cabinet, and the gas flow rate was adjusted to control the RH in the desiccator cabinet. The RH and temperature were monitored in real time using a thermohygrometer (P330, Swiss). We used the bubbler method to control the RH in high-humidity environments. Variations of resistance were measured using a two-probe method. Electromechanical stability of the samples as a function of the number of bending cycles was measured by repeated bending using a homemade bending system. A 2.0-cm-long CM underwent release bending for up to 10,000 cycles with simultaneous recording of electrical resistance.

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