Self-healing gate–based releasing mechanism
This protocol is extracted from research article:
Leaf-inspired homeostatic cellulose biosensors
Sci Adv, Apr 16, 2021; DOI: 10.1126/sciadv.abe7432

To quickly form a conductive path between the skin and the surface of the sensor when the CM is attached to the skin with dense hair layer (fig. S4, E and G), a self-healing gate–based releasing mechanism was developed. The perforated pore inside the CM is reopened by a contact pressure of 9.8 to 17.6 kPa generated when attaching the CS to the skin. At that time, approximately 20 to 30 μl of saline solution is released to the skin (fig. S3E). The saline penetrates into the dense hair layers (fig. S4E) to form a conductive interface between the skin and CS. The gate then recloses by the self-healing effect of the CM. If an extra electrolyte is required to maintain the electrical sensor-skin interface for long-term measurement, a small amount of saline solution can be supplied through the pore reopened by electrode-finger pressure (fig. S3D).

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