CSSP or CP NPs (300 nmol) were incubated with 10, 1, or 0 mM DTT in 30 ml of PBS (pH 7.4) [30% ethanol (v/v)] at 37°C (n = 3). The released CPT and PR104A were simultaneously determined by HPLC. In addition, the molecular weight change of prodrugs was measured by Agilent 1290-6545 Q-TOF (Agilent, USA) to investigate the release mechanism after CSSP NPs were incubated with 1 mM DTT for approximately 2 hours.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.