Tissue segments were homogenized with T-PER (ThermoFisher Scientific, Massachusetts, USA) and centrifuged at 10,000 rpm at 4°C. Then the homogenates were incubated in wells of a 96-well plate at 37°C with 4.2 mM tetramethylbenzidine (Serva, Heidelberg, Germany), 2.5 mM citrate, 5 mM NaH2PO4 and 1.18 mM H2O2 pH 5.0 at a final volume of 150 μl. After 5 minutes the reaction was terminated by adding 50 ml 0.18M H2SO4. Absorbance was read at 450 nm against blank wells. Results were adjusted for tissue sample protein content on Bradford assay (Sigma-Aldrich) and they were expressed as MPO units/mg protein/g.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.