Vero cells (ATCC) and 293-IQ cells (Microbix; HEK293 cells expressing the lac repressor [38]) were propagated at 37°C with 5% CO2 atmosphere in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5 or 10% Fetal Bovine Serum (FBS) and Penicillin-Streptomycin-L-Glutamine (PSG). Telomerized human fibroblasts stably expressing the coxsackie and adenovirus receptor (THF-CAR) were propagated in DMEM containing 10% FBS plus PSG. Aedes albopictus cells (C6/36 cells; ATCC CRL-1660) were propagated at 28°C with 5% CO2 in DMEM supplemented with 10% FBS and PSG.

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