The collection of putative lytic enzymes and their constituent domains starts with proteins that are already annotated with GO [75,76] terms associated with peptidoglycan binding and catalysis, e.g., “peptidoglycan binding”, “peptidoglycan endopeptidase activity”, and “peptidoglycan muralytic activity”. The current terms are provided in S2 Table. All identified sequences and their metadata are stored in the LEDGOs database. The sequences are clustered to 90% identity using CD-HIT [77], with the cluster representatives then serving as the seeds.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.