The measurement of intracellular Cl was performed as previously described [30]. Briefly, the primary cultured rat vaginal epithelial cells or the VK2/E6E7 cells were grown on glass cover slips with or without T. vaginalis infection for 3 h. The changes of fluorescence intensity of the Cl indicator dye, MQAE, was recorded using an imaging system (Olympus, IX83, Tokyo, Japan).

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