The measurement of intracellular Cl was performed as previously described [30]. Briefly, the primary cultured rat vaginal epithelial cells or the VK2/E6E7 cells were grown on glass cover slips with or without T. vaginalis infection for 3 h. The changes of fluorescence intensity of the Cl indicator dye, MQAE, was recorded using an imaging system (Olympus, IX83, Tokyo, Japan).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.