pGFP-C1-ERα (GFP-ERα), a mammalian expression of ERα fused to GFP, was purchased from Addgene (#28230; Watertown, MA, USA). Cultured MDA-MB-231 cells (2.5×104 cells/well in a 48-well plate; 2.5×105 cells/well in a 6-well plate) were transiently co- transfected with GFP-C1 (Dr. Jungeun An, Jeonbuk National University, Jeonju, South Korea), as a control construct, or GFP-ERα, using Lipofectamine™ 3000 (Invitrogen, #L3000008) together with control or CSNK1G2 siRNA as suggested by the manufacturer. Twenty four hours after transfection, the cells were treated with different combination of drugs.

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