pGFP-C1-ERα (GFP-ERα), a mammalian expression of ERα fused to GFP, was purchased from Addgene (#28230; Watertown, MA, USA). Cultured MDA-MB-231 cells (2.5×104 cells/well in a 48-well plate; 2.5×105 cells/well in a 6-well plate) were transiently co- transfected with GFP-C1 (Dr. Jungeun An, Jeonbuk National University, Jeonju, South Korea), as a control construct, or GFP-ERα, using Lipofectamine™ 3000 (Invitrogen, #L3000008) together with control or CSNK1G2 siRNA as suggested by the manufacturer. Twenty four hours after transfection, the cells were treated with different combination of drugs.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.