For molecular diagnosis, CRISPR genotyping, and dot blot hybridization, genomic DNA was obtained for all isolates from pure cultures using the E.Z.N.A.® Bacterial DNA Kit (Omega Bio-Tek, Carlsbad, CA, USA) following the manufacturer’s instructions. DNA quantification was carried out using the Qubit® 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, Massachusetts, USA).

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