TPC of the extract was determined according to the Folin-Ciocalteu method [26]. About 1 mg/mL of the sample solution was oxidized with 2 mL of 10% v/v Follin-Ciocalteu reagent (FCR) and 2 mL of 7.5% sodium carbonate. The mixture was incubated for 40 minutes at 45°C, and absorbance was measured at 765 nm using a UV/VIS spectrophotometer (JENWAY, UK). The TPC was calculated as a Gallic acid equivalent based on a standard calibration curve.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.