Standard laboratory markers were available for the total amount of 155 patients and were analyzed in the central laboratory at University Hospital RWTH Aachen using a Sysmex XN9000 (Sysmex GmbH, Norderstedt, Germany) and a Cobas 8000 c701 (Hoffmann-La Roche AG, Basel, Switzerland) platform according to the manufacturer’s instructions. For a small subset of patients (n = 17) serum samples upon ICU admission were available for serum concentration determination of markers beyond the clinical routine including adiponectin. Peripheral or central venous blood was obtained at ICU admission prior to any therapeutic intervention. Blood samples were centrifuged (2,000 x g for 10 minutes) and serum samples were stored at −80°C until use. Serum adiponectin levels were analyzed in a blinded fashion by an enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (No. EZHADP-61K, MERCK Millipore, Schwalbach, Germany).

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