For TEM, fresh root tip samples and leaf fragments were fixed in 2.5% glutaraldehyde for 3h, washed five times with 0.1 M sodium phosphate buffer (pH 7.0) and fixed into 1% OsO4 for 1 h. The samples were then completely desiccated in a series of ethanol (30 to 100%) and acetone, and processed further according to Zahra et al. [29].

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