The Na and K ions were measured according to Pii et al. [28]. Leaf and root samples were dried at 80°C and ground to fine powder by using grinding mill. A measured quantity of resulting powder was added 10 ml nitric acid and perchloric acid inside fume hood. The mixture was boiled on hot plate at 150–235°C following overnight incubation at room temperature until the formation of fumes. Then mixture was cooled for 2–4 min and rinsed with 3 ml distilled water. Samples were diluted with distilled water up to 50 ml. After filtration with Whatman paper, Na+ and K+ contents were estimated with a Shimadzu AA-680 atomic absorption flame spectrophotometer.

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