The MDA content/lipid peroxidation was assayed by measuring malendialdehyde according to the procedure described by Esfandiari et al. [24]. Plant samples were mixed in 2.5 ml of 5% trichloroacetic acid (TCA) and thiobarbitaric acid and 1.5 ml of crude enzyme extract. The homogenate was heated at 95°C for 15 min, chilled on ice and mixture was centrifuged at 4800 rpm for 10 min. Supernatant was collected and wavelength was recorded at 532 nm by deducting the non-specific absorption at 600 nm.

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