Chlorophyll contents were measured according to Gitelson et al. [23]. Briefly, fresh leaves were detached from the plants and chopped. Afterwards 10 ml of dimethyl sulfoxide (DMSO) was added to 0.05 g leaf samples. The resulting mixture was incubated at 65°C for 72 h in water bath. The extract obtained after incubation was centrifuged for 5–10 min at 7000 rpm and supernatant was collected. The supernatant was then read at 663 nm, 645 nm, and 480 nm wavelengths for determination of chlorophyll-a, chlorophyll-b and carotenoid, respectively.

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