Statistical analyses were performed with Prism, version 9 (GraphPad Software). Data are expressed as the mean ± standard deviation. Statistical variance between each experimental group was analysed using an analysis of variance test. Bartlett's test was used to determine homogeneity of variances. All tests were two‐tailed, and P‐values < 0.05 were considered statistically significant. We calculated the overall survival of rats in vivo experiments for lenvatinib dose optimization using the Kaplan‐Meier method. Analyses were conducted using EZR (Saitama Medical Center, Jichi Medical University), a graphical user interface of R version 2.13.0 (The R Foundation for Statistical Computing) and a modified version of R commander (version 1.6‐3) that includes statistical functions that are frequently used in biostatistics. 31

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