To assess accuracy, recovery tests were carried out by spiking mycotoxin-free corn powder with each mycotoxin standard solution at different concentrations. For AFB1 detection, the standard solution of AFB1 was spiked into a corn powder at final concentrations of 20, 30, 40, 60 and 80 μg/kg. For ZEN detection, the standard solution of ZEN was spiked into a corn powder at final concentrations of 100, 200, 300, 400 and 800 μg/kg. For DON detection, the standard solution of DON was spiked into a corn powder at final concentrations of 200, 400, 800, 1600 and 3200 μg/kg. Corn powder was used as a blank sample. Each sample was divided into 15 replicates, processed separately and subsequently analyzed. The procedures were carried out according to the UPT-LF, ELISA and HPLC/MS/MS methods described above. The recovery rate was expressed as the deviation of experimental from nominal concentration values in percent, recovery (%) = (measured value–blank value)/theorical value×100.

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