The limits of detection (LODs) of UPT-LF and HPLC-MS/MS were estimated according to the China National Standard (GB/T 5009.1–2003) [26]. Mycotoxin-free corn powder was used as a blank sample and was spiked at five different mycotoxin concentrations, with three replicates per concentration. The calibration curves were constructed by a linear regression analysis. The LOD of UPT-LF was calculated using the formula LODU = Ks/b, where K is a coefficient determined according to a certain degree of confidence and number of replicates, which can be obtained by checking the t-distribution quantile table in the China National Standard (GB/T 4889–2008) [27]; s is the standard deviation of the blank samples; and b is the slope of the calibration curve measured by UPT-LF. The LOD of HPLC-MS/MS was calculated as LODH = 3N/b, where N is the instrumental noise level and b is the slope of the calibration curve measured by HPLC-MS/MS. The LOD of ELISA was calculated as LODE = 0.01/b, where b is the slope of the calibration curve measured by ELISA.

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