VEGFA concentration in the cultured media from LX‐2 cells was measured using RayBio Human VEGFA ELISA Kit (RayBiotech, Inc) according to the manufacturer's instructions. A total of 1 × 106 LX‐2 cells were pre‐treated with PDGF‐BB (50 ng/mL) for 2 hours and then were treated with lenvatinib (0‐100 nmol/L) for 6 hours following overnight starvation.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.