Mouse serum IL-23, IL1α, TNF-α, IFNγ, MCP-1, IL-12p70, IL-1β, IL-10, IL-6, IL-27, IL-17A, IFNβ, and GM-CSF levels were analyzed using a multiplex beads-based assay (LEGENDplexTM) according to the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Serum PTH was measured using the mouse PTH (1–84) ELISA kit (Quidel, San Diego, CA) that detects the biologically active intact form of PTH.

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