To assess in vitro cell apoptosis, cleaved caspase‐3 and cleaved Poly (ADP‐ribose) polymerase (PARP) 1 concentrations in cell extract from LX‐2 cells were measured using Human Cleaved Caspase‐3 (Asp175) ELISA and Human Cleaved PARP1 ELISA kit (Abcam, Cambridge, UK), respectively, according to the manufacturer's instructions. A total of 1 × 106 LX‐2 cells were treated with lenvatinib (0‐200 nmol/L) for 24 hours following overnight starvation.

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