The TdT‐mediated dUTP Nick‐End Labelling (TUNEL) technique was used to evaluate neuronal apoptosis. The In Situ Cell Death Detection Kit (Roche, Switzerland, Germany, 11 684 795 960) was used according to the instruction. The nuclei were stained with DAPI, and fluorescent microscopy (Olympus, Tokyo, Japan) was used to evaluate neuronal apoptosis.

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