The relationship between the SPRY2 and miR‐27‐3p was verified on the database Tarcan. Next, the luciferase reporter plasmid containing wild‐type 3’‐UTR of SPRY2 was purchased from Genomeditech. Wild‐type and the mutated vectors were co‐transfected with miR‐27‐3p mimics or negative control into HEK‐293T cells using Lipofectamine2000. After 48 hours, Luciferase activity of cultured supernatant was measured using a kit as per the manufacturer's instructions.

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