The relationship between the SPRY2 and miR‐27‐3p was verified on the database Tarcan. Next, the luciferase reporter plasmid containing wild‐type 3’‐UTR of SPRY2 was purchased from Genomeditech. Wild‐type and the mutated vectors were co‐transfected with miR‐27‐3p mimics or negative control into HEK‐293T cells using Lipofectamine2000. After 48 hours, Luciferase activity of cultured supernatant was measured using a kit as per the manufacturer's instructions.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.