FcγRIIb-/- mice were provided by Dr. Silvia Bolland (NIAID, NIH, Maryland, USA) and housed at the Faculty of Medicine, Chulalongkorn University. All animal procedures were approved by the Institutional Animal Care and Use Committee at Faculty of Medicine, Chulalongkorn University. Mice were maintained in accordance with the Guide for the Care and Use of Laboratory Animals (eighth edition), National Research Council. They had free access to water and standard rodent chow (C.P. Mice Feed, Perfect Companion Group Co., Ltd., Thailand)

Male and female heterozygous (FcγRIIb+/-) mice were crossed to generate FcγRIIb-/- mice and their WT littermates. FcγRIIb-/- and WT controls were genotyped by PCR from tail biopsies as described earlier [15]. Three- and 6-month-old FcγRIIb-/- males and WT controls were used to evaluate a skeletal phenotype. They were anesthetized with isoflurane and sacrificed by cervical dislocation. Left mandibles were fixed in 10% neutral buffered formalin for microcomputed tomography (μCT) analysis.

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