A 22G EUS fine‐needle aspiration (FNA) (Wilson Cook, Winston‐Salem, NC) or fine‐needle biopsy (FNB) needle (SharkCore FNB needle Medtronic Corp., Boston, MA) was used to diagnose and stage a treatment‐naïve solid pancreatic mass with the aid of rapid on‐site evaluation. Once diagnostic material was confirmed, a dedicated pass was performed and snap frozen and stored at −80°C for subsequent DNA/RNA extraction. Cytologic smears were assessed for molecular testing adequacy metrics to include cellularity 15 DNA and RNA were co‐extracted using the Qiagen ALLprep kit (#80284) according to manufacturer's instructions and quantitated using the Qubit fluorometer (ThermoFisher #Q33327). Sanger sequencing was utilized to determine the G12 KRAS mutation status of each case from PCR amplicons as described previously. 9

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