Cells were grown in 12‐well plates until about 80‐90% confluency was reached and then a 10‐μL pipette tip was used to create a scratch/wound with clear edges across the width of a well. Wells were treated either with vehicle (DMSO) or 40 μmol/L sirtinol. Photographs were acquired with Olympus CKX53 microscope at 0 hours or 24 hours. All experiments were performed in triplicates.

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