The human HSC line, LX‐2, was purchased from Merck. The cells were washed, suspended in Dulbecco's modified Eagle's medium (DMEM) (Nacalai tesque) supplemented with 10% foetal bovine serum (FBS) (Gibco) and antibiotics (1% penicillin and streptomycin), plated on 24‐well plastic culture dishes and incubated at 37°C in a 5% CO2 air environment. 26 LX‐2 cells have been authenticated using Short Tandem Repeat profiling within the last 3 years. Mycoplasma testing was performed with MycoProbe® Mycoplasma Detection Kit (R&D Systems, Inc) according to the manufacturer's protocol. For some assays, cells were incubated with recombinant transforming growth factor‐β1 (TGF‐β1) (Sigma‐Aldrich), or PDGF‐BB (Sigma‐Aldrich) or basic FGF (bFGF) (R&D Systems) with or without different concentrations of lenvatinib (ChemScene).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.