Cells were incubated on uncoated glass coverslips for duration of 24 hours, and then fixed by means of 4% paraformaldehyde for duration of 15 minutes at room temperature. The adherent cells were permeated in PBS containing 0.1% Triton X‐100, followed by blocking with 1% bovine serum albumin (BSA)/PBS containing 10% normal goat serum for 1 hours and incubation overnight with primary anti‐rabbit antibodies (Abcam, Cambridge, UK) against α‐SMA (1:500, ab32575), FAP (1:500, ab53066), FSP‐1 (1:250, ab124805) and vimentin (1:500, ab193555) at 4°C. Afterwards, cells were incubated with Alexa Fluor® 647‐conjugated secondary donkey anti‐rabbit immunoglobulin G (IgG) antibody (ab150075, 1:500) for 1 hours. Finally, cells were fixed with Vectashield containing 4′6‐diamidino‐2‐phenylindole (DAPI) and observed under a LSCM.

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