Cells were subcultured in 6‐multi‐well plates (2.5 × 105 cells/well) for Western blot analysis or in 24‐multi‐well plates (0.5 × 105 cells/well) for cell viability assay. The next day, as recommended by manufacturer, cells were transfected with control siRNA (siRNA scrambled) or sirt1 siRNA (Thermo Fisher Scientific) in complete medium using lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) for a total of 72 hours.

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