Adrenocortical tumour cells (H295R and SW13 cells) were purchased from the American Type Culture Collection (ATCC, Rockville, MD). H295R cells were maintained as previously described. 29 SW13 were maintained in high glucose DMEM (Dulbecco's modified Eagle's medium) (Thermo Fisher Scientific, Monza, Italy) supplemented with 10% foetal bovine serum, 1% glutamine and 1% penicillin‐streptomycin (Sigma‐Aldrich Srl., Milan, Italy). All cells were maintained at 37˚C in a humidified atmosphere of 95% air and 5% CO2. Cell monolayers were subcultured into 6‐well plate for protein and RNA extraction (1 x 106 cells/plate), into 12‐multi‐well for colony formation (2 x 103 cells/well) and wound healing assay (3 x 105 cells/well) and into 48‐multi‐well for MTT assay (2 x 104 cells/well). Doubling time for SW13 cells is about 24 hours and for H295R cells is 48‐72‐hours: this difference has been considered in cell experiments. 30 H295R and SW13 cells were maintained in complete medium for 48 and 24 hours, respectively, and then treated with sirtinol (Sigma‐Aldrich) in complete medium.

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