5 × 103/wells MHCC97H and Hep3B cells were seeded in a 96‐well plate. Cells were transfected with indicated plasmids after being cultured for 24 hours. 20 μL MTS buffer (K300‐500, Amyjet Scientificlnc, China) was added to each well and incubated for 2‐4 hours after cells were transfected for 24 hours. The absorbance value was measured at 450 nm. Five duplicated wells were set for each group. Cell survival rates = (optical density (OD)EXP − ODEmpty/ODControl − ODEmpty)%.

The experiment was repeated 3 times.

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