CDX2‐3′‐untranslated region (3′UTR) was subjected to artificial synthesis and insertion into psiCHECK‐2 vector (Promega, Madison, WI, USA). Mutant (MUT) of complementary sequence of seed sequences was designed on the basis of CDX2 wild‐type (WT) sequence and inserted into psiCHECK‐2 vector. Two recombinant plasmids, namely CDX2‐WT and CDX2‐MUT, were co‐transfected into human embryonic kidney 293T (HEK‐293T) cells with miR‐24‐3p mimic and mimic NC. Subsequent to a 48‐hours transfection, the cells were lysed. Following this, luciferase activity was measured using Glomax 20/20 luminometer (Promega, Madison, WI, USA). The luciferase activity was directly measured by the ratio of firefly luciferase activity to Renilla luciferase activity.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.