CDX2‐3′‐untranslated region (3′UTR) was subjected to artificial synthesis and insertion into psiCHECK‐2 vector (Promega, Madison, WI, USA). Mutant (MUT) of complementary sequence of seed sequences was designed on the basis of CDX2 wild‐type (WT) sequence and inserted into psiCHECK‐2 vector. Two recombinant plasmids, namely CDX2‐WT and CDX2‐MUT, were co‐transfected into human embryonic kidney 293T (HEK‐293T) cells with miR‐24‐3p mimic and mimic NC. Subsequent to a 48‐hours transfection, the cells were lysed. Following this, luciferase activity was measured using Glomax 20/20 luminometer (Promega, Madison, WI, USA). The luciferase activity was directly measured by the ratio of firefly luciferase activity to Renilla luciferase activity.

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