Each group of cells were seeded in a 6‐well plate as 2.5 × 104/cm2. After cells were cultured for 24 hours, a scratch was made through the centre of each well using the 100 µL sterile pipette tip. The scratch was observed and imaged at the start and 24 hours following the scratch using a microscope. Image J software (1.48; National Institutes of Health) was used to measure the migrated distance. Cell migration capacity was evaluated by comparing scratch width. Rate of wound healing = (0‐width – 24‐width)/0‐width × 100%. Each group was set up with independent triplicates.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.