Formalin‐fixed paraffin‐embedded 5‐μm tissue sections were incubated at 60°C for 30 minutes and then deparaffinized in xylenes, rehydrated through a graded series of alcohol concentrations for 5 minutes and rinsed by water for 2 minutes. After antigen retrieval by 1mM Tris‐EDTA (pH = 8.0) was performed, all sections were rinsed with phosphate buffer saline (PBS) for three times and left to block at room temperature in 3% H2O2‐methanol for 10 minutes, followed by the coating of antibodies at room temperature for overnight at 4℃. Afterwards, the sections were rinsed three times in 0.1% PBST, incubated with an Enhancer buffer (PV‐9000, Zsbio, Beijing, China) for 20 minutes and incubated with enzyme‐labelled anti‐mouse/rabbit secondary antibodies (PV‐9000, Zsbio) for 30 minutes. Subsequently, the proteins were subjected to diaminobenzidine (DAB) development and left to counterstain with haematoxylin for 1 minute. After the differentiation and addition of blue coloration, the sections were dehydrated by gradient concentration ethanol, transparentized and sealed by neutral resins. Immunohistochemistry images were obtained using an upright microscope (BX53, OLYMPUS, Japan) and analysed and scored by experienced pathologists. Primary antibodies used: KDM5A (1:400, Abcam, Cambridge, UK, ab217292) and CD31 (1:400, Abcam, ab9498).

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