Hep3B cell line (BNCC289780) was obtained from BN Bio (Beijing, China). MHCC97H cell line (YS‐ATCC087) was obtained from Shanghai YSRIBIO industrial co., LTD. (Shanghai, China). Human embryonic liver cells HHL5 were obtained from Wuhan Weikesaisi Technology Co., Ltd. (Wuhan, China). Human amniotic epithelial cells (HAECs) were obtained from Mingzhou Bio (Ningbo, China). Human umbilical vein endothelial cells (HUVECs) were obtained from Keygen BioTECH (Nanjing, China). Hep3B, MHCC97H and HHL5 were cultured in Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Carlsbad, CA, USA) supplemented with 10% foetal bovine serum (FBS; Thermo Fisher Science, Inc, Waltham, MA, USA), 100 U/mL penicillin and 100 µg/mL streptomycin. HAECs and HUVECs were cultured in M‐199 medium supplemented with endothelial cell growth supplement: 100 U/mL penicillin and 100 µg/mL streptomycin. For the induction of angiogenesis, HUVECs or HAECs were stimulated by condition medium (50% supernatant from HepG3 + 50%M‐199 medium) after confluency reached 60%‐80% and normal HUVEC was as control. 3 × 105 cells were plated in a 6‐well plate and transfected with indicated plasmid at 50% confluence by using Lipofectamine 2000 Transfection Reagent (11668‐019, Invitrogen, Carlsbad, California, USA) by following the instructions from the manufacturer. Opti‐MEM (51985042, Gibco, Gaitherburg, MD, USA) was mixed with 4μg indicated plasmids or Lipofectamin2000 for 5 minutes at room temperature and were then mixed in a two‐regent well for 20minutes before being added to a 6‐well plate. After transfection, cells were cultured at 37°C in a saturated humidity atmosphere containing 95% air and 5% CO2; the medium was replaced after 6 hours, and lysates were collected after 48 hours.

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