110 cases of HCC biopsy specimens were collected at the Shanghai Tenth People's Hospital and confirmed by pathological diagnosis. None of the patients received prior radiotherapy and chemotherapy. 46 cases were over 65 years old. There were 64 cases under 65 years old; 61 cases with tumour diameters <5 cm and 49 cases with tumour diameters over 5 cm. Of these, 33 paired of cases of HCC and adjacent tissues were randomly selected for further study.

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