Migration assay was performed using 24‐well transwell migration Boyden chambers (8 μm pore size; Costar, Cambridge, MA, USA) pre‐coated with matrigel (dilution 1:2 in DMEM; BD Biosciences, Bedford, MA, USA) as reported. 4 Quantitative analysis was assessed counting for each sample the migrated cells in 10 microscopic fields (objective used: 20X) from three independent experiments. Results have been expressed as mean values ± SD. p values were calculated using Student's t test, and significance level has been defined as P > .05.

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