Migration assay was performed using 24‐well transwell migration Boyden chambers (8 μm pore size; Costar, Cambridge, MA, USA) pre‐coated with matrigel (dilution 1:2 in DMEM; BD Biosciences, Bedford, MA, USA) as reported. 4 Quantitative analysis was assessed counting for each sample the migrated cells in 10 microscopic fields (objective used: 20X) from three independent experiments. Results have been expressed as mean values ± SD. p values were calculated using Student's t test, and significance level has been defined as P > .05.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.